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The You Yangs support a rich variety of plant species. Tall eucalypts such as Manna Gum, Yellow Gum and River Red Gum give way to sparse undergrowth of native shrubs and groundcovers. Patches of Wattle and Drooping She oaks also occur.
The endangered Brittle Greenhood Orchid is one of thirty species of orchids to be found in the park. One pest plant which has become prominent is Boneseed, a native of South Africa. Today it chokes out large areas of native vegetation.
Parks Victoria is removing Boneseed from areas of high botanical value and supporting research into methods of biological control. Volunteer groups and schools are also actively involved with the boneseed control program.
The park provides a range of habitats for wildlife. More than 200 species of birds have been recorded in the park and koalas, kangaroos, wallabies, brush-tail possums, echidnas and lizards are commonly seen.