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The park’s sandy soils support 619 plant species representing nearly a quarter of Victoria's native flora. The ranges are special for their wildflowers. Many of the plants are rare, or remote from their normal localities in East Gippsland and the drier west of the state. The locally common Brisbane Ranges grevillea is found nowhere else in the world.
There are more than 180 bird species, which include the Peregrine Falcon, migratory Rainbow Bird and Powerful Owl. Koalas are particularly abundant around Anakie Gorge. Other mammals include Swamp Wallabies, Eastern Grey Kangaroos and the nocturnal Brush-tailed and Ring-tailed Possum, Sugar Gliders and Tuans.